PTU is a non-competivie inhibitor. The tube continuing the increase if substrate did not react. PTU binds to the allosteric site and changes the shape of the active site. It does not matter how much substrate is added, the reaction will not happen is PTU is present.Click to see full answer. Also to know is, what is the role of phenylthiourea PTU in the experiment?The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase. Phenoloxidase is a key enzyme of melanization catalyzing the oxidation of phenols. Phenylthiourea (PTU) is the well-known and widely used inhibitor of phenoloxidase. However, the mechanism of its action is not quite clearAlso, is lead a competitive inhibitor of catechol oxidase? Lead inhibits the catechol oxidase. This inhibition can be measured if the reduction in melanin production is quantified using a colorimeter or a SAPS colour chart. In this practical, students extract the catechol oxidase from banana and mix it with varying concentrations of lead which is thought to inhibit the enzyme. Keeping this in view, what is the role of copper ions in the catechol oxidase reaction? Catechol oxidase is a copper oxidase that contains a type 3 di-copper cofactor and catalyzes the oxidation of ortho-diphenols into ortho-quinones coupled with the reduction of molecular oxygen to water.How does a competitive inhibitor work?In competitive inhibition, an inhibitor that resembles the normal substrate binds to the enzyme, usually at the active site, and prevents the substrate from binding. Competitive inhibitors are commonly used to make pharmaceuticals. For example, methotrexate is a chemotherapy drug that acts as a competitive inhibitor.
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